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Permeability of Hydrophilic

Supervisors: Vladan Milovic Professor Per Artursson

SUMMARY

Investigations of the integrity and transport characteristics of 2/4/A1 cells
have been done in this report. The cell line was isolated from rat fetal
intestinal epithelial cells and transfected with thermolabile SV40 large T
antigen.

These cells proliferated at 33 °C, but eliminated the antigen and ceased
proliferating at a non-permissive temperature (39°C). At 39°C 2/4/A1 cells
started to differentiate but simultaneously the cells also underwent massive
cell death.

When cultured at 37°C these cells formed confluent and tight monolayers that
seemed to have paracellular transport characteristics similar to that of the
human intestine. Transmission electron microscopy confirmed the development of
multilayers at 33°C, monolayers at 37°C and defects in the cell layer due to
apoptosis at 39°C.

Different immunostainings of ZO-1, E-cadherin and vinculin confirmed formation
of tight and......

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Approximate Word Count: 3148
Approximate Pages: 13 (250 words per double-spaced page)

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