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Microbiology

Unknown Number 29
Enterobacter aerogenes

TESTS DONE

1. Gram Stain – Gram Negative Rod

2. SIM tube – motile, non-sulfur producing, indole negative

3. Simmon's Citrate tube – positive

4. Durham tubes –
a. Does ferment galactose
b. Does not ferment glucose
c. Does ferment maltose
d. Does ferment glycerol
e. Does ferment sucrose
f. Does ferment trehalose

5. Urea tube – urease negative

6. Oxidase test – does not produce cytochrome oxidase

7. Catalyse test – produced bubbles

8. Grew at 41۫C and not at 4۫C

9. Spirit Blue Agar – lipase positive

10. Lysine Decarboxylate – lysine negative

11. Nitrate Broth – nitrites produced (turned red after adding both reagents)

12. MR-VP medium –
a. Methyl red positive
b. Indole negative
c. Voges-Proskauer negative
d. Citrate utilization positive

According to Bergey's Manual, the Genus Enterobacter are straight rods, Gram negative. Most are motile and classified as......


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Approximate Word Count: 279
Approximate Pages: 2 (250 words per double-spaced page)

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