Purification Of Egg White Protein
Title: Purification of Egg white protein
Name: Michael Johnson
Partner: David Logad & Nandita
Date: 2nd – 9th September 2004
Group: Thursday 11:30am - 3:30pm
Introduction
Salting Out
In 1888 Hofmeister that it can be possible to dehydrate a protein by adding salt to the solution, salting out. When a protein in a aqueous solution it is surrounded by water, in fact there can be up to 0.35g of water tightly bound to 1g of protein (Simpson 2004).
Also the effectiveness of the salting out can be affected by the type of salt used. This is because of the charge the salt can bring on the protein making it either likely or less likely interacted with water.
"The protein structure can also have an affect with it hydophobicity with hydrophobic patches on it (caused by the side chains Phe, Tyr, Leu, Ile, Met & Val). So the optimum salt is one that will cause hydration of the polar regions and dehydration of the hydrophobic regions of the protein. With which the salt does not......
View the rest of this paper...
Approximate Word Count: 4310
Approximate Pages: 18 (250 words per double-spaced page)
Why should you join Frat Files?
- - It's safe, secure, and private.
- - Instant access to over 100,000 papers. New papers are added hourly.
- - Fast and reliable customer support.
Similar Essays
-
Purification Of Egg White Protein
Purification of Egg white protein. Title: Purification of Egg white protein
Name: Michael Johnson Partner: David Logad & Nandita ... -
Extraction And Characterization Of Proteins
... In the experiment, analytical protein purification method was used ... part was the
determination of protein concentration ... 20mL egg white was measured out and 1.0 M ... -
Extraction And Characterization Of Proteins
... by then was a dirty white, sour-smelling ... Warburg-Christian assay and the egg albumin
concentration ... a) Many compounds used in enzyme purification interfere with ... -
New Developments Or Research In Genetic Cloning: Summary
... they were genetically identical female white lambs. ... "Purification and Molecular Cloning
of Plx , a Cdc25 ... Regulatory Kinase from Xenopus Egg Extracts." Science ... -
New Developments Or Research In Genetic Cloning: Summary
... they were genetically identical female white lambs. ... "Purification and Molecular Cloning
of Plx , a Cdc25 ... Regulatory Kinase from Xenopus Egg Extracts." Science ...
Frat Files
Members
Information
