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Gene Cloning

Clone: A group of identical cells or organisms
To clone a gene:
1) Insert the gene into a vector that can carry the into a host cell
2) Ensure that it will replicate there
3) Insertion is carried out by cutting the vector and the DNA to be inserted with the same restriction endonuclease to ensure that both have the same "sticky ends"
Vector for cloning the bacteria come from two major types:
(1) Plasmids, and
(2) Phages
Recombinant DNA Technology
A) It was developed in 1970
B) Procedure:
(1) Obtain DNA from an organism
(2)ADD Restriction enzymes
(3) A reproducible set of fragments
(4)Inset into replicating DNA molecules
(5) This gives Recombinant DNA
(6) Introduce this DNA in appropriate cells
Why clone a gene?
Let's say you have a Genome with GeneA, GeneB & GeneC
Your gene of interest is GeneA, You would
(1) Select the gene of interest
(2) Clone the gene, which could be used for
a) DNA sequence, Protein product expression
b) Gene Therapy......

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Approximate Word Count: 418
Approximate Pages: 2 (250 words per double-spaced page)

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