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Reaction Rates of Barley Alpha Amylase with Starch at Specific pH's and Temperatures

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Abstract

Enzyme's are used as catalysts in certain reactions. They help lower the activation energy needed for the reaction to go to completion. At optimum temperature and pH the amount of collisions of substrate and enzyme is at its highest, any deviation from the optimum temperature and pH will result in the denaturization of the enzyme. The purpose of this experiment is to find the optimum temperature and pH for the reaction of Barley alpha-amylase and starch. The predicted optimum temperature and pH is 50-60 degrees Celsius and pH of 5-6. To test this hypothesis, a starch solution was mixed with the amylase and then tested for absorbance, using a spectrophotometer, at several different temperatures and pHs. From these experiments it was concluded that the optimum temperature was 55 degrees Celsius and the optimum pH was 5. these results lead to the conclusion that the Barley......

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Approximate Word Count: 1864
Approximate Pages: 8 (250 words per double-spaced page)

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