#11tlc
TLC Analysis of Analgesic Drugs #11
The purpose of this lab was to learn techniques 19 column chromatography sections 19.1-19.3 and technique 20 thin layer chromatography and to analysis analgesic drugs with thin layer chromatography, which showed all the different chemicals in the drugs.
Needed to make 12 capillary micropipettes to have small enough capillaries to spot solutions. Handle the TLC paper with care to prevent oils from your hands to get on the paper. Making the spots small prevent the spots from going in the paths of the other spots on the TLC paper. The TLC paper is placed in the developing chamber to allow the spots to develop. Then they are placed under a UV lamp to observe the spots that formed on the TLC paper. Then in millimeters the spots are measured relative to the solvent front. The iodine analysis is not preformed until after all the UV comparison is complete.
A major deviation was not being able to make the capillary micropipettes.
With spot......
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Approximate Word Count: 439
Approximate Pages: 2 (250 words per double-spaced page)
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