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Identification of Mystery Plasmids
Abstract
Plasmids serve as important tools in genetics and biotechnology labs, where they are commonly used make many copies of a particular gene. The aim of this experiment was to identify three mystery plasmids based upon their characteristics; such as size, antibiotic resistance, lacZ profile and conjugative properties. The results obtained showed that plasmid number2 was the pDSK519 plasmid and its size was 26229.58 Bp. Plasmid pDSK519 also was found to be resistant to kanamycin only and it possessed the LacZ gene. pDSK519 had the oriT gene but it did not have the tra gene. Plasmid number 22 was found to be pHSG298 as it had a size of 9767.08 Bp and it was found to be resistant to kanamycin only. It also possessed the LacZ gene and conjugation results showed that it did not possess the oriT gene the tra gene. Plasmid number 12 was found to be pRP1:: Tn501 as it was resistance to all three antibiotics. It was also found out that this plasmid......

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Approximate Word Count: 4010
Approximate Pages: 17 (250 words per double-spaced page)

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