Reaction Rates Of Barley Alpha-Amylase Enzyme With Substrate Starch At Different Ph’S And Temperatures
Abstract
Enzymes are proteins that lower the activation energy needed for chemical reactions. The two main environmental factors that can affect the enzyme’s activity are temperature and pH, and each enzyme works best at a particular temperature and pH. The purpose of this enzyme kinetic experiment was to observe the effect of temperature and pH on the reaction of barley alpha-amylase enzyme with starch substrate and establish the optimum temperature and pH for this reaction. The optimum temperature and pH for the reaction of alpha-amylase and starch was predicted to be a temperature of 50°C and a pH of 5. The optimum temperature and pH for the reaction was determined by monitoring the reaction rate of alpha-amylase at different temperatures and pH’s by means of using a spectrophotometer to measure the disappearance (in absorbance) of the substrate starch. As a result, the absorbance of the substrate starch decreased at different rates for each temperature and pH as time......
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Approximate Word Count: 1951
Approximate Pages: 8 (250 words per double-spaced page)
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