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Haematology

Blood Film Staining – Normal & Malignant Cells



Case Study 1

Male 72 yrs

Hb 77g/l (NR 130-180)
RBC 2.23 x 10¹²/l (NR 4.5-6.5)
MCV 88fl (NR 76-96)
WBC 98.2 x 10/l (NR 4-11)
Platelets 28 x 10/l (NR 150-400)

A) Calculation of PCV (packed cell volume or haematocrit)

PCV = RBC (cells/l) x 10¯¹² x MCV (fl)

RBC = 2.23 x 10¹²/l
MCV = 88fl or 88 x 10/l

PCV = 2.23 x 88 x10
PCV = 196.24/1000
PCV = 0.196 (NR 0.4-0.54)


B) Calculation of MCH (mean cell volume)

MCH = Hb (g/dl) x 10
RBS (cells/l) x 10¯¹²

Hb = 77g/l or 7.7g/dl
RBC = 2.23 x 10¹²/l

MCH = 7.7 x 10 =34.52 pg (10¯¹²g)/cell (NR 27-32 pg (10¯¹²g)/cell)
2.23



C) Calculation of MCHC (mean corpuscular haemoglobin concentration)

MCHC = Hb (g/dl)
PCV (l/l)

Hb = 77g/l or 7.7g/dl
PCV = 0.196

MCHC = 7.7 = 39.3g/dl or 393 g/l (NR 30-35 gt/dl)
0.196


The blood film shows large number of......


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Approximate Word Count: 1868
Approximate Pages: 8 (250 words per double-spaced page)

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