Frederick Douglass
Matthew Peachey
Immunology
3/4/02
Toolbox #5—Immunoprecipitation and Use of Antibodies in Isolation of Genes
One of the most useful discoveries of recent technology is the ability to isolate individual genes from an organism's entire genome and then identify that gene. There are several methods available to achieve this goal, many of which make use of antibodies to identify potions of molecules. For proteins such as cell surface proteins, it is very difficult to purify a protein solution. To make antibodies to these types of proteins, whole cells or cell mixtures are injected into rabbits and the antibodies later collected. The antibodies must be separated from the other types in sera however. To accomplish this, techniques such as affinity chromatography are used to isolate antibodies form sera. Isolated antibodies can then be added to a protein solution, allowing the binding of particular proteins causing precipitation from solution. This type of isolation of......
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