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Introduction:
The purpose of this lab was to measure the extent of enzyme reaction on given substrates by means of color change. The reaction followed is given below:
Tyrosinase„³
Enzyme
Pyrocatechol Hydroxyquinone
Oxidation/Reduction Pink „³ Brown

E+S + [ES] = E+P
Enzyme Reaction

Hypothesis: If there is an increase in enzyme concentration, an increase in reaction temperature, or an increase in buffer pH, then greater intensity in a given reaction will be experienced, resulting in greater manipulation of the final substrate product; measured by the extent of substrate color change.
Greater manipulation of substrate when introduced into stronger, less diluted enzyme, higher buffer pH, and/or higher temperature is expected.
We know that color change is an effective method of measuring chemical activity within a given reaction. The stronger, more drastic the color change, the more chemical activity......

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Approximate Word Count: 657
Approximate Pages: 3 (250 words per double-spaced page)

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