Gc/Ms
Purpose:
The Purpose of this experiment is to determine the quantity of caffeine in a sample using an instrument called Gas Chromatography-Mass Spectroscopy (GC-MS). The caffeine samples that are evaluated are coffee, espresso and a deuterated caffeine sample (d3), given by the T.A., of unknown concentration was used as an internal standard.
Theory:
The GC-MS instrument combines two different techniques to form one single method of analyzing mixtures. Gas chromatography performs the separation of components in a mixture, and the mass spectroscopy distinguishes the individual constituent.
A mixture of different specimens can be separated by GC. A sample is injected into the instrument through an injection port. The injection port is maintained at certain mixture, which is called its' mobile phase, to the stationary phase of the instrument. The stationary phase is the GC column, where each compound in the mixture interacts at different rates, and therefore separated. The......
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